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KMID : 1025520120540060383
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Journal of Animal Science and Technology
2012 Volume.54 No. 6 p.383 ~ p.394
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Gene Expression Profiling in Hepatic Tissue of two Pig Breeds
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Jang Gul-Won
Lee Kyung-Tai
Park Jong-Eun
Kim Hee-Bal
Kim Tae-Hun
Choi Bong-Hwan
Kim Myung-Jick
Lim Da-Jeong
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Abstract
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Microarray analyses provide information that can be used to enhance the efficiency of livestock production. For example, microarray profiling can potentially identify the biological processes responsible for the phenotypic characteristics of porcine liver. We performed transcriptome profiling to identify differentially expressed genes (DEGs) in liver of pigs from two breeds, the Korean native pigs (KNP) and Yorkshire pigs. We correctly identified expected DEGs using factor analysis for robust microarray summarization (FARMS) and robust multi-array average (RMA) strategies. We identified 366 DEGs in liver (p<0.05, fold-change>2). We also performed functional analyses, including gene ontology and molecular network analyses. In addition, we identified the regulatory relationship between DEGs and their transcription factors using in silico and qRT-PCR analysis. Our findings suggest that DEGs and their transcription factors may have a potential role in adipogenesis and/or lipid deposition in liver tissues of two pig breeds.
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KEYWORD
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Korean native pig, Yorkshire, Microarray, Molecular network, Transcription factor
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